Leader
Pamela Mellon, Ph.D.
Professor of Reproducive Medicine and Neuroscience
Phone: (858) 534-1140, e-mail: mellonadmin@ucsd.edu
Contact
Ella Kothari
E-mail: stemc@ad.ucsd.edu
For pronuclear microinjections and rederivation inquiries, e-mail juzhao@ucsd.edu.
For cryopreservation inquiries, e-mail hoakley@ucsd.edu.
For stem cell inquiries, e-mail ekothari@ucsd.edu.
Service profile
- Pronuclear microinjection of DNA to create transgenic mice
- Blastocyst microinjection of embryonic stem cells to create knockout mice
- Embryonic stem cell gene targeting for homologous recombination
- Ovary transplantation to rescue infertile mice
- Embryo rederivation to generate pathogen-free mice
Overview
The Transgenic Core and Embryonic Stem Cell shared resource is a state-of-the-art facility that has an outstanding track record in the production of genetically altered mice. Transgenic mice carrying new or novel genes are created by microinjection of DNA into the pronuclei of fertilized eggs. Knock-out mice lacking specific genes of interest are created by homologous recombination in embryonic stem cells followed by injection into blastocysts to create chimeric mice. Highly experienced personnel produce transgenic and knock-out mice for UCSD investigators at very reasonable cost and with very short lead times.
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