Flow Cytometry

Director

Thomas J. Kipps, MD, PhD
Professor of Medicine
Phone: 858-534-5400
Email: tkipps@ucsd.edu

Contact

Dennis Young
Phone: 858-822-0407
Fax: 858-822-0403
Email: djyoung@ucsd.edu

Location

UC San Diego Moores Cancer Center
3855 Health Sciences Drive, MC 0803
La Jolla, CA 92093-0803
Room 2317-Q

Service Profile

  • Aseptic sorting of living cell populations (up to 4 simultaneous collection tubes)
  • Direct cloning into microtiter tissue culture plates or onto microscope slides
  • Isolation of rare cell subpopulations or large number of cells
  • Quantitative fluorescence analysis (up to 9 colors)
  • Consulting (design of experiments; analysis or conversion of data)
  • Typical applications include:
    • Viability and toxicity assays
    • Apoptosis assays (TUNEL, sub-G1, caspases, Annexin V, mitochondrial membrane potential, etc)
    • Calcium Flux
    • Intracellular pH
    • Degranulation
    • Oxidative Burst
    • Phagocytosis
    • Reticulocytes
    • Platelets
    • Bead-based assays
  • AutoMACS
  • For more information, see Service Details

Equipment Profile

  • BD FACSAria (High speed, 9-color, triple-laser cell sorter)
    • Automated Cell Deposition Unit (ACDU)
    • 4-way sorting
  • BD FACSCalibur (2) - 4-color, non-sorting cytometers
  • Miltenyi AutoMACS

Overview

The speed at which the automated instruments of this resource can examine or separate free floating cell populations for several parameters - sometimes simultaneously - and chart the results, provides powerful opportunities for obtaining data on cell populations of statistically reliable size.

More information