Do you need to clean up a particular mouse line from pathogenic contamination? The Transgenic Core uses the following method for Embryo Rederivation:

Client coordinates the receipt of at least 2 males ages between 2-5 months, of desired strain into the Rodent Isolation Unit (RIU) with the Animal Care Program Aquisitions Office

Core aquires wild-type females of the appropriate background strain and schedules procedure

Core superovulates and RIU personnel mate females with males in RIU

Core collects embryos, washes them and transfers into the oviducts of pseudopregnant recipient females

Client receives pregnant recipient females into their animal colony approximately 4 days after procedure

Client separates mothers, records births, and genotypes offspring

Client provides a female mouse ideally less than 8 months old that is infertile or cannot carry pups to term

Core removes ovaries of donor female, bisects ovaries

Core ovariectomizes recipient females and transplants donor ovary into 3 females total - 1 Bilateral and 2 Unilateral

Core transfers recipients immediately back to Client

Client mates recipients with wild-type males 2 weeks after surgery

Client genotypes pups to ensure the donor ovary has contributed eggs

Do you need to store valuable lines of mice for future use? The Transgenic Core uses the following method for Embryo Cryopreservation:

Client coordinates the receipt of at least 8 males ages 2-5 months, of desired strain into the Rodent Isolation Unit (RIU) with the Animal Care Program Aquisitions Office

Core aquires wild-type females of the appropriate background strain and schedules procedure

Core superovulates and RIU personnel mate females with males in RIU

Core collects embryos, washes them then freezes them in vials using DMSO as cryoprotectant

Core will store frozen embryos a maximum of one week prior to transfering vials to the client for long term storage

Core thaws 1 vial of frozen embryos when required by investigator (all inclusive in the price of freezing) and cultures them to blastocyst stage

Core implants blastocysts into oviducts of pseudopregnant recipient female mice

Client receives pregnant recipient females into their animal colony approximately 4 days after procedure

Client separates mothers, records births, and genotypes offspring