SHARED RESOURCES - HISTOLOGY & IMMUNOHISTOCHEMISTRY

Website: http:// cancer.ucsd.edu/histology Leader: Nissi M. Varki, M.D. Professor of Medicine and Pathology 858-534-4933 / nvarki@ucsd.edu Contact: Laarni Gapuz, MT (ASCP) email: lgapuz@ucsd.edu Phone: (858) 822-1011 Location: Moores Cancer Center, 3855 Health Sciences Drive #0815, Fifth floor #5314

Advisory for Stem Cell Researchers Cancer Center Core rates will be different from published rates if the research involves cell lines other than those that appear on the NIH Human Pluripotent Stem Cell Registry. The NIH Human Pluripotent Stem Cell Registry is found at                         http://stemcells.nih.gov/ The Cancer Center facilities managers and technical personnel will not request this information from each user because it is the Principal Investigator's responsibility to follow all pertinent guidelines and laws for the appropriate conduct of research. Investigators are responsible for advising the shared resource director if the research involves pluripotent cell lines that are not on the NIH Human Pluripotent Stem Cell Registry. Please alert the facility manager if your research involves non-Registry cell lines and the service fee for Cancer Center members will be adjusted to the full, non-Cancer Center member rate. Facility personnel are happy to answer your questions.

Service Profile:

- Freezing and embedding of tissues for histology

- Frozen sections

- Paraffin blocks processing and Paraffin sections

- Hematoxylin and Eosin staining of sections

- Specialized histochemical staining

- Individualized immunohistochemical assays

- Specialized assays: TUNEL, BrdU, beta galactosidase, etc.

- Interpretation assistance; mouse histology and pathology
- Design and interpretation of immunohistology and in situ hybridization analysis

- Laser Capture Microdissection

 

Overview of the Shared Resource:

This facility allows a complete histologic phenotypic analysis of tissues from genetically altered mice; embryo to adult. It is indispensable for providing quality control for the presence of cancer in a human tissue sample prior to use in biochemical or molecular biological analysis. Conversely, it establishes the absence of tumor in tissue utilized as a normal control for similar experiments.

A research service of the NCI-designated Moores UCSD Cancer Center: http://cancer.ucsd.edu