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20X Phosphate Buffered Saline
(Dulbucco's w/o Ca+2 & Mg+2)
20X for a longer shelf life than
1X or even 10X.
20X PBS Stock Solution:
Dissolve in THIS ORDER (they'll dissolve faster) in 1 liter of H2O:
- 2.88 g Na2HPO4
- 160.00 g NaCl
- 4.00 g KH2PO4
- 4.00 g KCl
Adjust to pH 7.2 with 10 M NaOH. Filter and store at room temperature.
1X PBS Working Solution:
- Add 50 ml of 20X PBS Stock Solution to 950 ml glass distilled water.
(Optional) Add 1 g NaN3 and/or 20 g Bovine Serum Album.
- Mix well.
- Store at 4°C.
- Use cold buffer for diluting and washing cells.
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Dennis J.
Young, UCSD Cancer Center Flow Cytometry Core Facility |
