Website:

http:// cancer.ucsd.edu//flow

Leader:

Thomas J. Kipps, M.D., Ph.D.

Professor of Medicine

(858) 534-5400 / tkipps@ucsd.edu

Contact:

Name: Dennis Young

Phone: (858) 822-0407

Fax: (858) 822-0403

Email: djyoung@ucsd.edu

Location: Moores Cancer Center
3855 Health Sciences Drive mc0803
La Jolla CA 92093-0803
Room 2317-Q

Service Profile:

• Aseptic sorting of living cell populations (up to FOUR simultaneous collection tubes)
• Direct cloning into microtiter tissue culture plates or onto microscope slides
• Isolation of rare cell subpopulations or large number of cells
• Quantitative fluorescence analysis (up to 9 colors)
• Consulting (design of experiments; analysis or conversion of data)
• Typical applications include:
• Viability and toxicity assays
• Apoptosis assays (TUNEL, sub-G1, caspases, Annexin V, mitochondrial membrane potential, etc)
• Calcium Flux
• Intracellular pH
• Degranulation
• Oxidative Burst
• Phagocytosis
• Reticulocytes
• Platelets
• Bead-based assays
• AutoMACS

Equipment Profile:

• BD FACSAria (High speed, 9-color, triple-laser cell sorter)
• Automated Cell Deposition Unit (ACDU)
• 4-way Sorting
• BD FACSCalibur (2) – (4-color, non-sorting cytometers)
• Miltenyi AutoMACS

Overview of the Shared Resource:

The speed at which the automated instruments of this resource can examine or separate free floating cell populations for several parameters -- sometimes simultaneously -- and chart the results, provides powerful opportunities for obtaining data on cell populations of statistically reliable size.

A research service of the NCI-designated Moores UCSD Cancer Center: http://cancer.ucsd.edu