|
|
| SHARED RESOURCES - FLOW CYTOMETRY |
- Website:
- http:// cancer.ucsd.edu//flow
- Leader:
- Thomas J. Kipps, M.D., Ph.D.
- Professor of Medicine
- (858) 534-5400 / tkipps@ucsd.edu
- Contact:
- Name: Dennis Young
- Phone: (858) 822-0407
- Fax: (858) 822-0403
- Email: djyoung@ucsd.edu
-
- Location: Moores Cancer Center
3855 Health Sciences Drive mc0803
La Jolla CA 92093-0803
Room 2317-Q
|
Advisory for Stem Cell Researchers
Cancer Center Core rates will be different from published rates if the research involves cell lines other than those that appear on the NIH Human Pluripotent Stem Cell Registry. The NIH Human Pluripotent Stem Cell Registry is found at
http://stemcells.nih.gov/
Cancer Center facilities managers and technical personnel will not request this information from each user because it is the Principal Investigator's responsibility to follow all pertinent guidelines and laws for the appropriate conduct of research. Investigators are responsible for advising the shared resource director if the research involves pluripotent cell lines that are not on the NIH Human Pluripotent Stem Cell Registry. Please alert the facility manager if your research involves non-Registry cell lines and the service fee for Cancer Center members will be adjusted to the full, non-Cancer Center member rate. Facility personnel are happy to answer your questions.
|
Service Profile:
-
- Aseptic sorting of living cell populations (up to FOUR simultaneous collection tubes)
- Direct cloning into microtiter tissue culture plates or onto microscope slides
- Isolation of rare cell subpopulations or large number of cells
- Quantitative fluorescence analysis (up to 9 colors)
- Consulting (design of experiments; analysis or conversion of data)
- Typical applications include:
- Viability and toxicity assays
- Apoptosis assays (TUNEL, sub-G1, caspases, Annexin V, mitochondrial membrane potential, etc)
- Calcium Flux
- Intracellular pH
- Degranulation
- Oxidative Burst
- Phagocytosis
- Reticulocytes
- Platelets
- Bead-based assays
- AutoMACS
- Equipment Profile:
-
- BD FACSAria (High speed, 9-color, triple-laser cell sorter)
- Automated Cell Deposition Unit (ACDU)
- 4-way Sorting
- BD FACSCalibur (2) – (4-color, non-sorting cytometers)
- Miltenyi AutoMACS
- Overview of the Shared Resource:
- The speed at which the automated instruments of this resource can
examine or separate free floating cell populations for several parameters
-- sometimes simultaneously -- and chart the results, provides powerful
opportunities for obtaining data on cell populations of statistically
reliable size.
A research service of the NCI-designated
Moores UCSD Cancer Center: http://cancer.ucsd.edu
|
