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SHARED
RESOURCES - FLOW
CYTOMETRY
- Website:
- http://
cancer.ucsd.edu//flow
- Leader:
- Thomas J. Kipps, M.D., Ph.D.
- Professor of Medicine
- (858) 534-5400 / tkipps@ucsd.edu
- Contact:
- Name: Dennis Young
- Phone: (858) 822-0407
- Fax: (858) 822-0403
- Email: djyoung@ucsd.edu
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- Location: Moores Cancer Center
3855 Health Sciences Drive mc0803
La Jolla CA 92093-0803
Room 2317-Q
- Service Profile:
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- Aseptic sorting of living cell populations (up to FOUR simultaneous collection tubes)
- Direct cloning into microtiter tissue culture plates or onto microscope slides
- Isolation of rare cell subpopulations or large number of cells
- Quantitative fluorescence analysis (up to 9 colors)
- Consulting (design of experiments; analysis or conversion of data)
- Typical applications include:
- Viability and toxicity assays
- Apoptosis assays (TUNEL, sub-G1, caspases, Annexin V, mitochondrial membrane potential, etc)
- Calcium Flux
- Intracellular pH
- Degranulation
- Oxidative Burst
- Phagocytosis
- Reticulocytes
- Platelets
- Bead-based assays
- AutoMACS
- Equipment Profile:
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- BD FACSAria (High speed, 9-color, triple-laser cell sorter)
- Automated Cell Deposition Unit (ACDU)
- 4-way Sorting
- BD FACSCalibur (2) – (4-color, non-sorting cytometers)
- Miltenyi AutoMACS
- Overview of the Shared Resource:
- The speed at which the automated instruments of this resource can
examine or separate free floating cell populations for several parameters
-- sometimes simultaneously -- and chart the results, provides powerful
opportunities for obtaining data on cell populations of statistically
reliable size.
A research service of the NCI-designated
Moores UCSD Cancer Center: http://cancer.ucsd.edu
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