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SHARED RESOURCES - DNA SEQUENCING
Website:
             
http:// cancer.ucsd.edu/dna
Leader:
Brinda Rana, Ph.D. Assistant Adjunct Professor of Psychiatry
(858) 822-4010
bkrana@ucsd.edu
Contact:
Name: Oanh Hong Phone: (858) 822-2204 Email: ohong@ucsd.edu
Location: La Jolla Campus East, Moores Cancer Center, Room 5314, MC 0815
 

Advisory for Stem Cell Researchers

Cancer Center Core rates will be different from published rates if the research involves cell lines other than those that appear on the NIH Human Pluripotent Stem Cell Registry. The NIH Human Pluripotent Stem Cell Registry is found at
                    http://stemcells.nih.gov/ 
 

The Cancer Center facilities managers and technical personnel will not request this information from each user because it is the Principal Investigator's responsibility to follow all pertinent guidelines and laws for the appropriate conduct of research. Investigators are responsible for advising the shared resource director if the research involves pluripotent cell lines that are not on the NIH Human Pluripotent Stem Cell Registry.   Please alert the facility manager if your research involves non-Registry cell lines and the service fee for Cancer Center members will be adjusted to the full, non-Cancer Center member rate. Facility personnel are happy to answer your questions. 

Service Profile:

- ABI Prism® BigDye™ Terminator DNA Sequencing
- Custom Reagents and PCR Reactions for Difficult Templates
- ABI GeneScan® Microsatellite Sizing and SNP Identification
- Free Access to ABI Prism® GeneMapper™ Software for Fragment Analyses
- Free Access to ABI Primer Express™ Software for Primer Design
- 24-hour Access to Data via internet-based LIMS system
- Technical Advice and User Instruction
Equipment Profile:
2 Applied Biosystems 3130xl Genetic Analyzers
Overview of the Shared Resource:
Using state of the art equipment, this resource provides high-quality, inexpensive DNA sequencing, DNA fragment sizing, and SNP identification with Applied Biosystems Genetic Analyzers. Consultation and education are also available for users who experience difficulties optimizing their DNA template preparations and sequencing results.

A research service of the NCI-designated Moores UCSD Cancer Center: http://cancer.ucsd.edu


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