SHARED RESOURCES - MICROSCOPY

Website:

http://cancer.ucsd.edu/mic

Example Images:

http://vis.sdsc.edu/research/cancer.html

Leader:

James R. Feramisco, Ph.D.

Professor of Medicine

jferamisco@ucsd.edu

Contact:

Name: James R. Feramisco, Ph.D.

Phone: (858) 534-7287

Fax: (858) 534-1422

Location: La Jolla Campus - Cellular & Molecular Medicine Building, East, Lower Floor

Service Profile:

-Conventional, Confocal and Deconvolution Light Microscopy

-Image Analysis and Photoproduction

-Fluorescence In Situ Hybridization and Chromosomal Painting

-Cell Preparation and Fluorescence Staining

-3-D Digital Image Capture (still and time lapse) and Analysis

-Training & Consultation

-Enhanced Tissue Imaging

-Access to Needle Microinjection Equipment

-Enhanced 3-D Perspective Views in Collaboration with SDSC

Equipment Profile:

-Delta Vision Deconvolution Microscope System (Nikon TE-200 Microscope)

-Hamamatsu Cooled CCD Video-Camera connected to a Zeiss Axiophot

-Zeiss LSM510 Laser Scanning Confocal Microscope

-Xenogen IVIS 200 system for live animal bioluminescence and fluorescence imaging

-Nikon TE300 inverted fluorescence microscope with z-stepping motor

-Nikon E600 upright fluorescence microscope with SPOT RT color camera

-Nikon E600 teaching microscope with SPOT QE camera

-Eppendorf Semi-Automatic Cell Microinjector

-Unix PC and Macintosh Graphics Workstations

-Software Packages & Color Printers

          - iCys Research Imaging Cytometer

Overview of the Shared Resource:

Provides a group of specialized techniques and rare equipment to examine cellular functions of candidate genes. Offers expensive and powerful image-capture technology, in lapsed or real time, which can be critical to achieve basic research objectives of today's laboratory. Interfaces with the VisLab of the San Diego Supercomputer Center to provide enhanced 3-D perspective views of data sets. (see http://vis.sdsc.edu/research/cancer.html ).

The DeltaVision and Zeiss LSCM are housed in the new SOM Microscopy Facility along with the Neurosciences Microscopy Core (see http://microscopy.ucsd.edu for more details)

How to Access the Facility:

For access to the above equipment, contact Kersi Pestonjamasp at

(858) 822-3895/(858) 534-8803, kpestonjamasp@ucsd.edu,

or Steve McMullen at (858) 822-4187, swmcmullen@ucsd.edu.

For IVIS 200 contact Joseph Aguilera at (858) 822-0253, jaguilera@ucsd.edu.

For creating enhanced 3-D perspective views using MeshViewer, Volume Explorer, and other visualization tools offered by the VisLab, contact Colin McGinn at cmcginn@ucsd.edu.
For access to the iCys Research Imaging Cytometer, contact Joseph Aguilera at (858) 822-4180, jaquilera@ucsd.edu

Location:

Cellular & Molecular Medicine East,Basement

           DeltaVision deconvolution system & Zeiss LSM510 confocal

Moores Cancer Center Vivarium - IVIS 200

Moores Cancer Center room 2304 - Nikon upright and teaching microscopes

Moores Cancer Center room 5317 - Nikon inverted microscope

Online calendars:

http://calendar.yahoo.com/feramiscolab for the deltavision deconvolution microscope

http://calendar.yahoo.com/zeisslsm510confocal for the Zeiss LSM510 laser confocal

http://calendar.yahoo.com/ccivis200 for the Xenogen whole animal imaging system

http://calendar.yahoo.com/ccmicroscope for Nikon inverted/upright/teaching microscopes

http://calendar.yahoo.com/ccicys for iCys imaging cytometer

A research service of the NCI-designated Moores UCSD Cancer Center: http://cancer.ucsd.edu