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| SHARED RESOURCES - MICROSCOPY |
- Website:
- http://cancer.ucsd.edu/mic
- Example Images:
- http://vis.sdsc.edu/research/cancer.html
- Leader:
- James R. Feramisco, Ph.D.
- Professor of Medicine
- jferamisco@ucsd.edu
- Contact:
- Name: James R. Feramisco, Ph.D.
- Phone: (858) 534-7287
- Fax: (858) 534-1422
- Location: La Jolla Campus - Cellular & Molecular Medicine Building, East, Lower Floor
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Advisory for Stem Cell Researchers
Cancer Center Core rates will be different from published rates if the research involves cell lines other than those that appear on the NIH Human Pluripotent Stem Cell Registry. The NIH Human Pluripotent Stem Cell Registry is found at
http://stemcells.nih.gov/
The Cancer Center facilities managers and technical personnel will not request this information from each user because it is the Principal Investigator's responsibility to follow all pertinent guidelines and laws for the appropriate conduct of research. Investigators are responsible for advising the shared resource director if the research involves pluripotent cell lines that are not on the NIH Human Pluripotent Stem Cell Registry. Please alert the facility manager if your research involves non-Registry cell lines and the service fee for Cancer Center members will be adjusted to the full, non-Cancer Center member rate. Facility personnel are happy to answer your questions.
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Service Profile:
- -Conventional, Confocal and Deconvolution Light Microscopy
- -Image Analysis and Photoproduction
- -Fluorescence In Situ Hybridization and Chromosomal Painting
- -Cell Preparation and Fluorescence Staining
- -3-D Digital Image Capture (still and time lapse) and Analysis
- -Training & Consultation
- -Enhanced Tissue Imaging
- -Access to Needle Microinjection Equipment
- -Enhanced 3-D Perspective Views in Collaboration with SDSC
- Equipment Profile:
- -Delta Vision Deconvolution Microscope System (Nikon TE-200 Microscope)
- -Hamamatsu Cooled CCD Video-Camera connected to a Zeiss Axiophot
- -Zeiss LSM510 Laser Scanning Confocal Microscope
- -Xenogen IVIS 200 system for live animal bioluminescence and fluorescence imaging
- -Nikon TE300 inverted fluorescence microscope with z-stepping motor
- -Nikon E600 upright fluorescence microscope with SPOT RT color camera
- -Nikon E600 teaching microscope with SPOT QE camera
- -Eppendorf Semi-Automatic Cell Microinjector
- -Unix PC and Macintosh Graphics Workstations
- -Software Packages & Color Printers
- - iCys Research Imaging Cytometer
- Overview of the Shared Resource:
- Provides a group of specialized techniques and rare equipment to examine cellular functions of candidate genes. Offers expensive and powerful image-capture technology, in lapsed or real time, which can be critical to achieve basic research objectives of today's laboratory. Interfaces with the VisLab of the San Diego Supercomputer Center to provide enhanced 3-D perspective views of data sets. (see http://vis.sdsc.edu/research/cancer.html ).
- The DeltaVision and Zeiss LSCM are housed in the new SOM Microscopy Facility along with the Neurosciences Microscopy Core (see http://microscopy.ucsd.edu for more details)
- How to Access the Facility:
- For access to the above equipment, contact Kersi Pestonjamasp at
- (858) 822-3895/(858) 534-8803, kpestonjamasp@ucsd.edu,
- or Steve McMullen at (858) 822-4187, swmcmullen@ucsd.edu.
- For IVIS 200 contact Joseph Aguilera at (858) 822-0253, jaguilera@ucsd.edu.
- For creating enhanced 3-D perspective views using MeshViewer, Volume Explorer, and other visualization tools offered by the VisLab, contact Colin McGinn at cmcginn@ucsd.edu.
For access to the iCys Research Imaging Cytometer, contact Joseph Aguilera at (858) 822-4180, jaquilera@ucsd.edu
- Location:
- Cellular & Molecular Medicine East,Basement
- DeltaVision deconvolution system & Zeiss LSM510 confocal
- Moores Cancer Center Vivarium - IVIS 200
- Moores Cancer Center room 2304 - Nikon upright and teaching microscopes
- Moores Cancer Center room 5317 - Nikon inverted microscope
- Online calendar:
- http://microscopy.ucsd.edu/schedule
A research service of the NCI-designated Moores UCSD Cancer Center: http://cancer.ucsd.edu
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